The unusual transhydrogenase of Entamoeba histolytica
Open Access
- 12 January 2001
- journal article
- Published by Wiley in FEBS Letters
- Vol. 488 (1-2) , 51-54
- https://doi.org/10.1016/s0014-5793(00)02386-3
Abstract
We have expressed and purified a protein fragment from Entamoeba histolytica. It catalyses transhydrogenation between analogues of NAD(H) and NADP(H). The characteristics of this reaction resemble those of the reaction catalysed by a complex of the NAD(H)‐ and NADP(H)‐binding subunits of proton‐translocating transhydrogenases from bacteria and mammals. It is concluded that the complete En. histolytica protein, which, along with similar proteins from other protozoan parasites, has an unusual subunit organisation, is also a proton‐translocating transhydrogenase. The function of the transhydrogenase, thought to be located in organelles which do not have the enzymes of oxidative phosphorylation, is not clear.Keywords
This publication has 34 references indexed in Scilit:
- A Mitochondrion in Entamoeba histolytica?Parasitology Today, 2000
- Protein–protein recognition, hydride transfer and proton pumping in the transhydrogenase complex11Because Structure with Folding & Design operates a ‘Continuous Publication System’ for Research Papers, this paper has been published on the internet before being printed (accessed from http://biomednet.com/cbiology/str). For further information, see the explanation on the contents page.Structure, 2000
- Proton translocating nicotinamide nucleotide transhydrogenase from E. coli. Mechanism of action deduced from its structural and catalytic propertiesBiochimica et Biophysica Acta (BBA) - Bioenergetics, 2000
- Catalytic Properties of Hybrid Complexes of the NAD(H)-Binding and NADP(H)-Binding Domains of the Proton-Translocating Transhydrogenases from Escherichia coli and Rhodospirillum rubrumBiochemistry, 1998
- Structural and Catalytic Properties of the Expressed and Purified NAD(H)- and NADP(H)-Binding Domains of Proton-Pumping Transhydrogenase from Escherichia coliBiochemistry, 1997
- Proton-translocating Nicotinamide Nucleotide TranshydrogenaseJournal of Biological Chemistry, 1995
- Kinetic resolution of the reaction catalysed by proton‐translocating transhydrogenase from Escherichia coli as revealed by experiments with analogues of the nucleotide substratesEuropean Journal of Biochemistry, 1994
- Crosslinking and radiation inactivation analysis of the subunit structure of the pyridine nucleotide transhydrogenase of Escherichia coliBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1990
- Mitochondrial energy-linked pyridine nucleotide transhydrogenaseBiochemistry, 1973
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970