PREPARATION OF A STABLE HUMAN PLASMA PROTEIN SOLUTION 1

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Abstract
By the use of cold ethanol-water mixtures to remove the more unstable fibrinogen and globulin fractions from plasma a human plasma protein solution which is stable to heat was obtained. Electrophoretic analyses show the protein distribution to be approximately 80% albumin, 14% gamma-globulin and 6% beta-globulin. Optical density of the solution changes only slightly following heating at 60[degree]C for 10 hours. Ultracentrifuge analyses show that heating the solution for 10 hours at 60[degree]C causes the formation of a small amount of fast moving component. Very little, if any of this component is observed in the unheated solution.