Synchronous Reinitiation of Chromosome Replication in E. coli B/r after Nalidixic Acid Treatment
- 1 June 1970
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 66 (2) , 365-369
- https://doi.org/10.1073/pnas.66.2.365
Abstract
Nalidixic acid has been used to interrupt DNA synthesis in exponentially growing cultures of E. coli B/r. Within three minutes after removal of the nalidixic acid block, premature rounds of chromosome replication are initiated at the normal origin of vegetative DNA synthesis. The new growing forks traverse the chromosome in about 40 minutes, the normal replication time. These rounds continue synchronously into the second generation after removal of the nalidixic acid. These phenomena are similar in some respect to the premature initiation of chromosomal replication observed after thymine starvation or after release of thermo-sensitive DNA synthesis mutants from the restrictive temperature that prevents DNA synthesis.Keywords
This publication has 8 references indexed in Scilit:
- Initiation of DNA replication cycles in Escherichia coli following DNA synthesis inhibitionJournal of Molecular Biology, 1969
- ORIGIN AND DIRECTION OF DNA SYNTHESIS IN E. coli B/rProceedings of the National Academy of Sciences, 1969
- The origin and direction of replication of the chromosome of Escherichia coli B-r.Proceedings of the National Academy of Sciences, 1968
- DNA synthesis during the division cycle of rapidly growing Escherichia coliJournal of Molecular Biology, 1968
- On the Bacterial Life SequenceCold Spring Harbor Symposia on Quantitative Biology, 1968
- Revised linkage map of Escherichia coli.1967
- Premature chromosome replication induced by thymine starvation: Restriction of replication to one of the two partially completed replicasJournal of Molecular Biology, 1965
- Induction of replication by thymine starvation at the chromosome origin in Escherichia coliJournal of Molecular Biology, 1964