Monoclonal Antibodies Against Human Placental Glutathione Transferase (Class п )
- 1 February 1991
- journal article
- research article
- Published by Mary Ann Liebert Inc in Hybridoma
- Vol. 10 (1) , 89-94
- https://doi.org/10.1089/hyb.1991.10.89
Abstract
Five monoclonal antibodies (MAbs) were produced in a mouse hybridoma system against human placental glutathione transferase (GST п). Four of these monoclonal antibodies, named 461 to 464, were of immunoglobulin G class, whereas the monoclonal antibody 465 was of IgA class. All these MAbs specifically recognized the glutathione transferase from human placenta (class п ) showing no cross reactivity against the basic and the neutral forms of GST from human liver. When each MAb was incubated with the GST п, no inhibition of enzymatic activity towards 1-chloro-2,4-dinitrobenzene was observed except for MAb 465 which showed a slight inhibition to a serial dilution of 1:128.Keywords
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