Screening method for rapid detection of methicillin-resistant (heteroresistant) Staphylococcus aureus
- 1 November 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 22 (5) , 886-887
- https://doi.org/10.1128/jcm.22.5.886-887.1985
Abstract
A broth screening method was developed to detect methicillin-resistant (heteroresistant) strains of Staphylococcus aureus from primary plates. Lyophilized vials containing cation-supplemented Mueller-Hinton broth, 2% NaCl, and 10 .mu.g of methicillin and 6 .mu.g of oxacillin per ml were hydrated and tested with 129 methicillin-resistant and 35 methicillin-susceptible strains of S. aureus. With the addition of a tetrazolium indicator after 5 h of incubation, 96.9% of resistant strains were detected. No false-positive results occurred.This publication has 1 reference indexed in Scilit:
- Successful use of broth microdilution in susceptibility tests for methicillin-resistant (heteroresistant) staphylococciJournal of Clinical Microbiology, 1983