Comparative Studies of the Binding of Dimeric and Monomeric Enkephalins to Neuroblastoma-Glioma NG108?15 Cells

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Abstract
Binding activity of the enkephalin dimer [D-Ala2,Leu5-NH-CH2-]2 (DPE2) to NG108-15 hybrid cells was compared to that of the monomer [D-Ala2,Leu5]enkephalin amide (DALEA). At 25.degree. C, the values of the apparent affinity constant for DPE2, measured to intact and lysed cells and membranes, was 5.0 (.+-. 0.09) .times. 109 M-1 for n = 28 experiments, as compared to 0.9 (.+-. 0.08) .times. 109 M-1 (n = 16) for DALEA. At 4.degree. C, the binding affinity of DPE2 decreased by 43% and that of DALEA by 33%. An important difference between the binding of DPE2 and DALEA was that, after necessary corrections for difference in maximal bindability of the respective tritiated enkephalins, the molar binding capacity for DALEA was 2-fold higher than for DPE2, although mutual cross-displacement studies indicated that binding occurred to 1 class of noninteracting homogeneous receptors. The binding capacity for intact and lysed cells and membranes was 20 (.+-. 2) .times. 10-11 M for DPE2 and 43 (.+-. 2) .times. 10-11 M for DALEA. The enkephalin monomers [D-Ala2,D-Leu5]enkephalin (DADLE) and [D-Ala2,Met5]enkephalin amide (DAMEA) showed binding characteristics similar to those of DALEA.