cDNA cloning and expression of polygalacturonaseinhibiting proteins (PGIPs) from red raspberry (Rubus idaeus)
Open Access
- 1 June 1997
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Experimental Botany
- Vol. 48 (6) , 1185-1193
- https://doi.org/10.1093/jxb/48.6.1185
Abstract
Plant polygalacturonase-inhibiting proteins (PGIPs) inhibit endo-polygalacturonases (endo-PGs) released by invasive fungi. Two potential PGlP cDNAs have been cloned from raspberry (Rubus idaeus). PGIP1 corresponds to a full-length PGIP cDNA with a high degree of identity with previously isolated genes and maintains all the characteristic features of PGIP peptides. A genomic fragment of PGIP1 identifies a single 243 bp intron which is efficiently spliced out of the PGIP1 premRNA transcript. PGIP2 contains a frame shift mutation which would lead to the loss of 105 amino acids off the C-terminus. Contrary to PGIP activity levels in raspberry (Johnston et al., 1993), expression analysis detected PGIP1 at equal levels throughout fruit development from closed flower, through to ripe fruit.Keywords
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