Evaluation of the Electrostatic Interaction Parameter on a Simulated Protein Titration Curve

Publisher Website
Google Scholar
Abstract
Values for the electrostatic interaction parameter, .omega., derived from the potentiometric titration curves of proteins may be erroneous unless both the number of groups ionizing with the same ionization constants are known and the titration curve is obtained with great precision and accuracy. The nature of the errors which can occur in the evaluation of .omega. is demonstrated for calculated curves constructed without electrostatic interaction assumed. Since in a protein, the number of groups (and their pK''s) titrating in a given pH region is unknown and the accuracy of protein titrations is limited, the titration of a protein can apparently be adequately described as the sum of a series of mass-law expressions.