A New Simple Plastic Chemotaxis Device of the Boyden Chamber Type Utilizing an Immunoassay Plate

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Abstract
A new simple and economical plastic Boyden chamber for in vitro assay of leukocytes chemotaxis was devised by the use of a commercially available immunoassay plate. Zymosan-activated rat serum, formyl-methionyl-leucyl-phenylalanine and leukotriene B4 caused concentration-dependent migration of the leukocytes into the lower chamber. About 50% of the cells loaded in the upper chamber migrated into the lower chamber for 80 min at the optimal concentrations of the three chemoattractants tested.