Effect of Various ions on Atp Determinations Using the “Luciferine-Luciferase” System

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Abstract
(6 figures) Various salts and buffers used in routine as part of the ATP extraction procedures induce an important inhibition of the peak light emission produced by the “luciferine-luciferase” system. The nature of the anion is more important in determining the inhibitory effect than the nature of the cation. The series obtained when placing the anions studied by order of increasing effectiveness is as follows Ac−C1−I−C104−. KCIO4 appears thus as a strong inhibitor of the enzyme activity. It appears moreover to act competitively with respect to ATP, one mole of inhibitor binding per mole of ATP active site. These results are discussed in connection with the use of the “luciferine-luciferase” system for ATP and other energy-rich compounds' concentration measurements.