Identification of phytoplasmas in eggs, nymphs and adults of Scaphoideus titanus Ball reared on healthy plants
- 1 May 1997
- journal article
- Published by Wiley in Insect Molecular Biology
- Vol. 6 (2) , 115-121
- https://doi.org/10.1111/j.1365-2583.1997.tb00079.x
Abstract
A survey for the presence of aster yellows‐related phytoplasmas in the different life stages of Scaphoideus titanus was carried out by means of PCR and nested‐PCR assays. Using a phytoplasma 16Srl group‐specific primer pair followed by RFLP analysis of amplified products, we were able to detect and identify phytoplasmas from eggs, newly hatched nymphs, fourth and fifth instar nymphs and adults reared on ten phytoplasma‐free Vicia faba seedlings. Two of these broad beans became infected, whereas PCR failed to detect phytoplasmas in the same ten plants before leafhopper rearing. These results suggest the possibility of transovarial transmission of aster yellows‐related phytoplasmas in S. titanus.Keywords
This publication has 25 references indexed in Scilit:
- 10.1007/BF00304715Published by Test accounts ,2011
- Mixed Infection of Grapevines in Northern Italy by Phytoplasmas Including 16S rRNA RFLP Subgroup 16SrI-B Strains Previously Unreported in This HostPlant Disease, 1996
- Detection of Multiple Phytoplasmas in Perennial Fruit Trees with Decline Symptoms in ItalyPhytopathology®, 1995
- Classification of plant-pathogenic mycoplasma-like organisms using restriction-site analysis of PCR-amplified 16S rDNAJournal of General Microbiology, 1993
- Detection and Differentiation of Plant-Pathogenic Mycoplasmalike Organisms Using Polymerase Chain ReactionPhytopathology®, 1993
- Development in Cladonia ochrochloraMycologia, 1993
- Colchis TodayGeographical Review, 1993
- Sensitive detection and identification of mycoplasma-like organisms in plants by polymerase chain reactionsBiochemical and Biophysical Research Communications, 1992
- Group Testing for Estimating Infection Rates and Probabilities of Disease TransmissionPhytopathology®, 1985
- SOME HOST PLANTS OF A CANADIAN ISOLATE OF THE CLOVER PHYLLODY VIRUSCanadian Journal of Plant Science, 1967