Induced Protein Synthesis during the Adaptation to H2 Production in Chlamydomonas moewusii

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Abstract
Gas production by Chlamydomonas moewusii in the light has been followed by manometric techniques during the adaptation to anaerobiosis. The only detectable gases produced are CO2 and H2 CO2 is produced at a rather constant rate whereas H2 evolution increase with time. This increase of H2 evolution during the adaptation period can be inhibited by cycloheximide and by chloral hydrate, two inhibitors of protein synthesis. If the inhibitors are added to already adapted cells there is no effect on H2 evolution. Adapted cell suspensions are sensitive to oxygen. Incubation under O2 for 10 min inhibits the H2 evolution to 100%. After removal of oxygen the capability to evolve H2 can be restored only by a new adaptation period. This second adaptation to H2 evolution can also be inhibited by cycloheximide.