The use of the Gouy diffusiometer with dilute protein solutions. An assessment of the accuracy of the method

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Abstract
The construction, use, and possible accuracy of a slightly modified form of Gouy diffusiometer are descr.; results with bovine serum albumin show that an accuracy of [plus or minus] 1% may be obtained down to concns. of 0.2% or less, using only 1 or 2 ml. of soln. A short series of ultracentrifuge expts. lead to a value of the sedimentation constant of bovine serum albumin, 4.31 [plus or minus] 0.07 S, somewhat lower than the present accepted value. The diffusion and sedimentation results were combined to give a molecular weight of 65,360 for this protein Auth. summ.