A Study of the CopF Repressor of Plasmid pAMβ1 by Phage Display
- 15 May 2000
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 182 (10) , 2973-2977
- https://doi.org/10.1128/jb.182.10.2973-2977.2000
Abstract
We studied DNA binding of a transcriptional repressor, CopF, displayed on a filamentous phage. Mutagenesis of a putative helix-turn-helix motif of CopF and of certain bases of the operator abolished the protein-DNA interaction, establishing the elements involved in CopF function and showing that phage display can be used to study repressor proteins.Keywords
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