Possible catalytic activity of DNA in the reaction between the antitumor drug cis-diamminedichloroplatinum(II) and the intercalator N-methyl-2,7-diazapyrenium.
- 1 May 1991
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 88 (9) , 3516-3519
- https://doi.org/10.1073/pnas.88.9.3516
Abstract
The platinum(II) complex cis-[Pt(NH3)2(N7-N-methyl-2-diazapyrenium)Cl]2+ formed in the reaction between cis-diamminedichloroplatinum(II) and N-methyl-2,7-diazapyrenium reacts with N7 of guanine residues in DNA. The resulting adduct is kinetically inert within single-stranded DNA. Within double-stranded DNA, it is kinetically inert in 1 M NaClO4 and becomes labile as the salt concentration is decreased. Two products, cis-[Pt(NH3)2(N7-N-methyl-2-diazapyrenium)H2O]3+ and N-methyl-2,7-diazapyrenium, are released. The conformation of the platinated DNA is different in low- and high-salt conditions as shown by the chemical probe diethyl pyrocarbonate. These results are discussed in relation with a possible catalytic role played by the double-stranded DNA.Keywords
This publication has 13 references indexed in Scilit:
- Formation of a DNA monofunctionalcis-platinum adduct cross-linking the intercalating drug N-methyl-2, 7-diazapyreniumNucleic Acids Research, 1990
- Chemical and biological properties of a new series of cis-diammineplatinum(II) antitumor agents containing three nitrogen donors: cis-[Pt(NH3)2(N-donor) Cl]+Journal of Medicinal Chemistry, 1989
- REACTIVITY OF MONOFUNCTIONAL CIS-PLATINUM ADDUCTS AS A FUNCTION OF DNA-SEQUENCE1988
- Chloroacetaldehyde reacts with Z-DNAJournal of Molecular Biology, 1988
- The formation, isolation and characterization of DNA adducts produced by anticancer platinum complexesPharmacology & Therapeutics, 1987
- Reaction of nucleic acids and cis-diamminedichloroplatinum(II) in the presence of intercalating agents.Proceedings of the National Academy of Sciences, 1986
- Reevaluation of interaction of cis-dichloro(ethylenediamine)platinum(II) with DNABiochemistry, 1986
- Diethyl pyrocarbonate: a chemical probe for secondary structure in negatively supercoiled DNA.Proceedings of the National Academy of Sciences, 1985
- Chemical probes of DNA conformation: detection of Z-DNA at nucleotide resolutionCell, 1985
- Binding of cis - and trans -dichlorodiammineplatinum(II) to the nucleosome coreProceedings of the National Academy of Sciences, 1979