BINDING OF CORTICOSTEROIDS BY PLASMA PROTEINS. II. PAPER ELECTROPHORESIS AND EQUILIBRIUM PAPER ELECTROPHORESIS 12

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Abstract
Standard paper electrophoresis failed to indicate the association of corticosterone-4-C14 with bovine plasma albumin, human albumin (Fraction V) or with any separate protein component of normal human plasma. Preliminary dialysis of the protein solution was studied against the electrophoretic buffer containing either corticosterone-4-C14 or hydrocortisone-4-C14. The buffer outside the dialysis bag was used to impregnate paper for electrophoresis. This technique has allowed the demonstration of the electrophoretic migration of the bound corticosteroid with the albumin component of normal plasma. The affinity of corticosterone-4-C14 and hydrocortisone-4-C14 was greatest for normal human plasma, intermediate for human albumin (Fraction V), and least for bovine plasma albumin.