Formation of cobalt-free corrinoids in Rhodopseudomonas spheroides.
- 1 January 1977
- journal article
- research article
- Published by Center for Academic Publications Japan in Journal of Nutritional Science and Vitaminology
- Vol. 23 (3) , 179-185
- https://doi.org/10.3177/jnsv.23.179
Abstract
Co-free corrinoids (CFC) were found in the cells of R. sphaeroides grown without Co salt. A predominant fraction of 3 CFC on paper-electrophoregram was further purified, and investigated physico-chemically and microbiologically in comparison with that isolated previously from Chromatium. The electrophoretic and spectrophotometric patterns of CFC from R. sphaeroides were partly different from those of Chromatium CFC. The former CFC was a competitive inhibitor of cyanocobalamin in Ochromonas malhamensis. The CFC from R. sphaeroides differs from that of Chromatium in chemical structure, and the compound has an anti-vitamin B12 activity in O. malhamensis.This publication has 3 references indexed in Scilit:
- Cobalt-free corrinoids as vitamin B12 antagonistsArchives of Biochemistry and Biophysics, 1968
- Vitamin B12 in photosynthetic bacteria and methionine synthesis by Rhodopseudomonas spheroidesBiochemical Journal, 1967
- A vitamin B12 compound containing no cobalt.Proceedings of the National Academy of Sciences, 1965