Evaluation of Repetitive Element Sequence-Based PCR as a Molecular Typing Method for Clostridium difficile
- 1 June 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 41 (6) , 2454-7
- https://doi.org/10.1128/jcm.41.6.2454-2457.2003
Abstract
Repetitive element sequence-based PCR (rep-PCR) is a typing method that enables the generation of DNA fingerprinting that discriminates bacterial strains. In this study, we evaluated the applicability of rep-PCR in typing Clostridium difficile clinical isolates. The results obtained by rep-PCR were compared with those obtained by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping. A high correspondence between pattern differentiations produced by rep-PCR and PFGE was observed, whereas PCR ribotyping showed a lower level of discriminatory power.Keywords
This publication has 42 references indexed in Scilit:
- Evaluation of methods for molecular typing and identification of members of the genusBrevibacteriumand other related speciesFEMS Microbiology Letters, 2002
- Molecular typing and long-term comparison of Clostridium difficile strains by pulsed-field gel electrophoresis and PCR-ribotypingJournal of Medical Microbiology, 2001
- Adhesion of Clostridium difficile to Caco-2 cell line, a colonization factorReviews in Medical Microbiology, 1997
- Comparison of arbitrarily-primed polymerase chain reaction, restriction enzyme analysis and pulsed-field gel electrophoresis for typing Clostridium difficileJournal of Microbiological Methods, 1996
- ERIC sequences: a novel family of repetitive elements in the genomes of Escherichia coli, Salmonella typhimurium and other enterobacteriaMolecular Microbiology, 1991
- Distribution of repetitive DNA sequences in eubacteria and application to finerpriting of bacterial enomesNucleic Acids Research, 1991
- A novel repeated DNA sequence located in the intergenic regions of bacterial chromosomesNucleic Acids Research, 1990
- Nosocomial Clostridium difficile colonisation and diseaseThe Lancet, 1990
- Nosocomial Acquisition ofClostridium difficileInfectionNew England Journal of Medicine, 1989
- Repetitive extragenic palindromic sequences: A major component of the bacterial genomeCell, 1984