Spectrophotometric Methods for Monitoring the Microbial Transformation of Steroids
Open Access
- 1 January 1970
- journal article
- research article
- Published by American Society for Microbiology in Applied Microbiology
- Vol. 20 (2) , 251-253
- https://doi.org/10.1128/aem.20.2.251-253.1970
Abstract
A method has been devised for the determination of 9α-fluorohydrocortisone and 9α-fluoro-16α-hydroxyhydrocortisone in fermentation broths. The method involves extraction of the two steroids from the broth with ethyl acetate, separation through the formation of the water-soluble borate complex of the vicinally hydroxylated steroid, and estimation of each steroid spectrophotometrically.Keywords
This publication has 0 references indexed in Scilit: