Enzymatic Hypoglycemia in Alloxan Diabetic Rats.

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Abstract
Summary Trypsin and chymotrypsin, administered intraperitoneally as single doses of 146 mg/kg to fed diabetic rats, are able to lower blood sugar. Covalent derivatives of active-site serine in both enzymes, and of active-site histidine in chymotrypsin, are hypoglycemically inactive. Trypsin and chymotrypsin produce hypoglycemia as a result of their actions in stimulating cellular uptake of sugar and conversion of glucose to glycogen in muscle. The recent demonstration of the proteolytic nature of insulin, and the complete elucidation of the amino acid sequence of chymotrypsin allow the formulation of predictions concerning the mechanism of action of insulin.