Bourgeonnement in vitro a partir d'épiderme séparé de feuille de Bryophyllum Daigremontianum (Crassulacées)

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Abstract
The lower epidermis from leaves of B. daigremontianum Berger was mechanically separated and cultured in liquid or solid medium; cytokinin and auxin in combination were necessary to obtain a lamellar callus, with the Murashige and Skoog''s macroelements being more efficient than White''s, Knop''s, or Lin and Staba''s. Numerous buds were initiated after 3 or 4 wk, especially with naphthaleneacetic acid (1 mg/l) in combination with 6-benzylaminopurine (0.2 mg/l). The best results for bud formation were obtained with the epidermis of the 1st and 2nd well-developed leaves. The buds, rooted in vitro, developed into normal plants. The histological study demonstrated that the callus originated only from the banal epidermal cells. The stomatic cells were not activated and died. The other tissues of the leaf never produced a bud in the same culture conditions; then, as for other species, the epidermis had a greater ability to initiate a budding program.

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