Low Incidence of Point Mutation of c-Ki-rasand N-rasOncogenes in Human Hepatocellular Carcinoma
- 1 March 1989
- journal article
- research article
- Published by Wiley in Japanese Journal of Cancer Research
- Vol. 80 (3) , 196-199
- https://doi.org/10.1111/j.1349-7006.1989.tb02290.x
Abstract
We examined the incidence of point mutation in codons 12, 13 and 61 of c-Ki-ras and N-ras genes in human hepatocellular carcinoma (HCC) using the polymerase chain reaction and oligonucleotide hybridization techniques. Among 34 tissue specimens surgically resected from 30 patients and 5 cell lines of human HCC, only two had ras point mutations; in one case, codon 12 of c-Ki-ras was altered from GGT, coding glycine, to GTT, coding valine; in the other case, codon 61 of N-ras was altered from CAA, coding glutamine, to AAA, coding lysine. Thus, point-mutational activation of ras oncogenes is an uncommon event in human HCC.Keywords
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