Reduction of acetylene by stationary cultures of free-living Rhizobium sp. under atmospheric oxygen levels

Abstract
The reduction of acetylene to ethylene by stationary (non-shaking) cultures of free-living rhizobia under atmospheric O2 levels was demonstrated. Under these conditions the development of the activity is inhibited by 10 mM NH4Cl and about 20% of O2 is required for maximal activity. When the stationary cultures were shaken, O2 concentrations of 1% and higher were inhibitory. Specific activities of 20 and 40 nmol of acetylene reduced h-1 mg-1 protein were observed.

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