Chemical Modification of Ribosomes with Dimethyl Sulfate: a Probe to the Structural Organization of Ribosomal Proteins and RNA

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Abstract
Ribosomal proteins from [14C]dimethyl sulfate treated 30S and 50S subunits of Escherichia coli ribosomes were separated by two-dimensional polyacrylamide gel electrophoresis and the degree of methylation of each protein was determined. Comparison of the results from this relatively non-specific chemical modification procedure with results from the milder lysine-specific reductive alkylation procedure reported previously (Moore, G. &Crichton, R. R. (1974) Biochem. J. 143, 607–612) has permitted a topographical classification of ribosomal proteins in terms of 'degree of exposure' in the 30S and 50S subunits.The reaction of dimethyl sulfate with ribosomal RNA, both in intact subunits and after isolation from the subunits, has indicated that approximately half of the RNA in 30S and 50S subunits is exposed on the surface of the ribonucleoprotein complexes, and that no large sections (extended sequences) of 16S RNA are concealed in the 30S subunit. It is proposed that modification of ribosomes with dimethyl sulfate is a potentially useful technique for probing exposed and hidden regions and also exposed single-stranded regions of RNA in ribosomes.

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