Isolation and properties of an isocitrate dehydrogenase from Anacystis nidulans
- 1 July 1981
- journal article
- research article
- Published by Springer Nature in Archiv für Mikrobiologie
- Vol. 129 (5) , 331-334
- https://doi.org/10.1007/bf00406456
Abstract
A NADP+-specific isocitrate dehydrogenase (EC 1.1.1.42) was isolated and purified over 400-fold from Anacystis nidulans. The enzyme activity responded slowly to rapid changes in ligand (NADP+, isocitrate, Mg2+-ions) or enzyme concentration as well as to rapid changes in temperature. These are properties characteristic of the hysteretic enzymes. In addition, the enzyme activity was subject to product (α-ketoglutarate) inhibition. ATP, ADP and CDP also inhibited the enzyme. Unlike several other cyanobacterial enzymes, the isocitrate dehydrogenase of Anacystis is not under redox control.Keywords
This publication has 24 references indexed in Scilit:
- Redox modulation of glucose‐6‐P dehydrogenase in Anacystis nidulans and its ‘uncoupling’ by phage infectionFEBS Letters, 1981
- Virus infection affects the molecular properties and activity of glucose-6-P dehydrogenase inAnacystis nidulans, a cyanobacteriumFEBS Letters, 1979
- Generic Assignments, Strain Histories and Properties of Pure Cultures of CyanobacteriaMicrobiology, 1979
- PHOTOTROPHIC PROKARYOTES: THE CYANOBACTERIAAnnual Review of Microbiology, 1977
- Molecular polymorphism and mechanisms of activation and deactivation of the hydrolytic function of the coupling factor of oxidative phosphorylationBiochemistry, 1976
- Influence of substrates and coenzymes on the role of manganous ion in reactions catalyzed by pig heart triphosphopyridine nucleotide-dependent isocitrate dehydrogenaseBiochemistry, 1976
- Nicotinamide adenine dinucleotide phosphate linked isocitrate dehydrogenase. Catalytic activation by the reduced coenzyme product of the reactionBiochemistry, 1976
- Control of NADP+‐specific isocitrate dehydrogenase from acinetobacter by nucleotidesFEBS Letters, 1976
- The Incomplete Tricarboxylic Acid Cycle in the Blue-green Alga Anabaena VariabilisJournal of General Microbiology, 1969
- The Metabolism of Acetate by the Blue-green Algae, Anabaena variabilis and Anacystis nidulansJournal of General Microbiology, 1967