Detection of respiratory syncytial virus in nasopharyngeal secretions by a biotin‐avidin ELISA more sensitive than the fluorescent antibody technique
- 1 February 1986
- journal article
- research article
- Published by Wiley in Journal of Medical Virology
- Vol. 18 (2) , 113-117
- https://doi.org/10.1002/jmv.1890180203
Abstract
The presence of respiratory syncytial virus (RSV) was investigated by immunofluorescent antibody (IF) technique and by a biotin/avidin (BA) ELISA in 156 samples of nasopharyngeal secretions (NPS) obtained from infants and small children with acute respiratory disease. Of 70 RSV-IF-positive NPS, 68 were positive by BA-ELISA. Of 86 RSV-IF-negative NPS, eight were positive by BA-ELISA. BA-ELISA could detect 0.5–1.0 ng RSV-protein.Keywords
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