Abstract
Monoclonal mouse hybridoma antibodies to human IgG subclasses 1–4 were used in an imprint immunofixation (IIF) technique to characterize the IgG subclass of a panel of IgG myeloma and normal sera separated by thin‐layer electrofocusing. The results show that the antisera were IgG subclass‐specific by this technique and that IIF can be a useful method in identifying IgG subclasses of IgG myeloma proteins in whole sera.