Synthesis of isotopically chiral [13C]penciclovir (BRL 39123) and its use to determine the absolute configuration of penciclovir triphosphate formed in herpes virus infected cells

Abstract
Isotopically chiral [13C]penciclovir (BRL 39123) has been synthesised via a stereospecific hydrolysis catalysed by the lipase from Candida cylindraceae and has been used to determine, by 13C NMR, that the triphosphate of penciclovir formed in herpes simplex type 1 infected cells has (S) stereochemistry with an enantiomeric purity of > 95%.

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