Protection from Oxygen Radical Damage in Human Diploid Fibroblasts by Acetyl-L-Carnitine

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Abstract
Oxygen radical production is suspected of being a major cause of aging. We have studied the effect of acetyl-L-carnitine (ALC) on oxygen metabolites toxicity in fibroblast cell lines derived from skin biopsies taken from apparently normal subjects. Fibroblast damage was produced by the generation of oxygen metabolites during the enzymatic oxidation of acetaldehyde with 50 mU of xanthine oxidase (XO). We measured lactate dehydrogenase (LDH) activity in the culture medium and cell viability in fibroblast cultures, both 4 days preincubated with 50 µg/ml ALC and not treated, after a 2-hour XO incubation in order to quantify the cell damage. We found a significant increase of LDH activity in cells without ALC exposed to free radical formation. No significant increase of the LDH activity was observed in the same cell lines, in the same experimental conditions after 4 days’ preincubation with 50 µg/ml ALC.

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