The amino acid sequence previously attributed to a protein kinase or a TCP1‐related molecular chaperone and co‐purified with phytochrome is a β‐glucosidase
- 20 June 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 347 (1) , 51-54
- https://doi.org/10.1016/0014-5793(94)00503-6
Abstract
A 60 kDa protein (P60) co‐purified with phytochrome was identified as avenacosidase, a β‐glucosidase which is part of the defense system of Avena sativa. An antiserum raised against P60 was used to isolate a cDNA clone coding for the complete amino acid sequence of P60. The cDNA‐derived amino acid sequence contained the partial sequences described before for a protein kinase [(1989) Planta 178, 199–206] and for a TCP1‐related molecular chaperone [(1993) Nature 363, 644–647] co‐purified with phytochrome. We conclude that these activities were related to minor contaminants and that only sequences of avenacosidase had been obtained.Keywords
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