Reactivity of Human Lymphoid and Lymphoblastoid Cells with Peanut Agglutinin: Detection of a Blood Cell Subset which Lacks Detectable Membrane HLA

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Abstract
The number of cells reacting with flurescein-labelled peanut agglutinin varies widely among human lymphoid organs. By separation techniques, reactive cell from tonsils were characterized as E-negative, non-immunoglobulin-bearing lymphocytes. In peripheral blood, the lectin reacted with a small percentage of lymphoid and monocytoid cells which lacked HLA membrane determinants but expressed the β2-mieroglobulin antigens. The tatter phenotype was also found on human lymphoblastoid cell lines. Lectin binding thus provides an effective means of characterizing these cell subsets.