INVOLVEMENT OF BOTH SYN-DIHYDRODIOL-EPOXIDE AND ANTI-DIHYDRODIOL-EPOXIDE IN THE BINDING OF 7,12-DIMETHYLBENZ(A)ANTHRACENE TO DNA IN MOUSE EMBRYO CELL-CULTURES
- 1 January 1983
- journal article
- research article
- Vol. 43 (7) , 3212-3218
Abstract
7,12-Dimethylbenz[a]anthracene (DMBA):deoxyribonucleoside adducts, from enzymic hydrolyses of DNA from mouse embryo cells exposed in culture to [3H]DMBA, can be separated into 2 fractions on the basis of whether or not they bind to the phenyl boronic acid residues of Servacel DHB. This suggests that these 2 fractions of adducts are derived from anti and syn bay-region dihydrodiol-epoxides, respectively. The fluorescence spectra and interactions of the major components of these 2 fractions with borate ions substantiate this interpretation. These findings indicats that both syn- and anti-dihydrodiolepoxides make a substantial contribution to DMBA binding to DNA in mouse embryo cells. For a given mouse embryo cell preparation, the relative contributions of each of these dihydrodiol-epoxides to DNA binding did not vary substantially with DMBA dose, with incubation time with DMBA, or in growing vs. confluent cultures, although there were differences between one cell preparation and another. [DMBA is a carcinogen.].This publication has 18 references indexed in Scilit:
- DNA and RNA adducts formed in hamster embryo cell cultures exposed to benzo[a]pyreneBiochemistry, 1978
- STUDY CHEMICAL CARCINOGENESIS .8. TUMOR-INITIATING ACTIVITY OF FLUORINATED 5-METHYLCHRYSENES1978
- Differences between products of binding of 7,12-dimethylbenz[a]anthracene to DNA in mouse skin and in a rat liver microsomal systemBiochemical and Biophysical Research Communications, 1978
- Cell and microsome mediated binding of 7, 12-dimethylbenz(a)anthracene to DNA studied by fluorescence spectroscopyCancer Letters, 1978
- Evidence for the involvement of a diol-epoxide in the binding of 7,12-dimethylbenz(a)anthracene to DNA in cells in cultureChemico-Biological Interactions, 1977
- Photosensitivity of dna‐bound 7,12‐dimethylbenz(a)‐anthraceneInternational Journal of Cancer, 1977
- The nature of benzo(a)pyrene-DNA adducts formed in hamster embryo cells depends on the length of time of exposure to benzo(a)pyreneBiochemical and Biophysical Research Communications, 1977
- Metabolic activation of the carcinogen 7,12-dimethylbenz[a]anthracene for DNA bindingBiochemical and Biophysical Research Communications, 1977
- Excision repair of benzo[a]pyrene-deoxyguanosine adducts in baby hamster kidney 21/C13 cells and in secondary mouse embryo fibroblasts C57BL/6J.Proceedings of the National Academy of Sciences, 1977
- (+/-)-7alpha,8beta-dihydroxy-9beta,10beta-epoxy-7,8,9,10-tetrahydrobenzo(a)-pyrene is an intermediate in the metabolism and binding to DNA of benzo(a)pyrene.Proceedings of the National Academy of Sciences, 1976