Production of parathyroid hormone‐related protein by cultured human myeloma cells

Abstract
We examined whether or not parathyroid hormone‐related protein (PTH‐rP) is produced in cultured human myeloma cells. PTH‐rP protein was detected in the cell extract of one (U266) of 6 myeloma cell lines examined by radioimmunoassay. Also we demonstrated the expression of PTH‐rP mRNA in all of the 6 lines. These findings indicate that PTH‐rP may be one of the factors responsible for the bone destruction or hypercalcemia in multiple myeloma.