Metabolism of Some Polyols byRhizobium meliloti

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Abstract
The utilization ofd-mannitol,d-arabitol, andd-sorbitol byRhizobium melilotiwas studied in extracts from mannitol-grown cells. Two different polyol dehydrogenases were induced by any of these polyols: (i) a nicotinamide adenine dinucleotide (NAD)-arabitol dehydrogenase and (ii) a NAD-sorbitol dehydrogenase, whereas polyol phosphate dehydrogenases were absent.d-Arabitol dehydrogenase was observed to act on bothd-arabitol andd-mannitol, butd-sorbitol dehydrogenase acted specifically ond-sorbitol.d-Arabitol was oxidized tod-xylulose,d-mannitol andd-sorbitol were oxidized tod-fructose. An adenosine triphosphate-linked hexokinase which acts ond-fructose and absence of hexose isomerase were also detected in this organism.