Mth1 receives the signal given by the glucose sensors Snf3 and Rgt2 in Saccharomyces cerevisiae‡
- 1 January 2000
- journal article
- research article
- Published by Wiley in Molecular Microbiology
- Vol. 35 (1) , 161-172
- https://doi.org/10.1046/j.1365-2958.2000.01688.x
Abstract
We have determined that the mutant genes DGT1‐1 and BPC1‐1, which impair glucose transport and catabolite repression in Saccharomyces cerevisiae, are allelic forms of MTH1. Deletion of MTH1 had only slight effects on the expression of HXT1 or SNF3, but increased expression of HXT2 in the absence of glucose. A two‐hybrid screen revealed that the Mth1 protein interacts with the cytoplasmic tails of the glucose sensors Snf3 and Rgt2. This interaction was affected by mutations in Mth1 and by the concentration of glucose in the medium. A double mutant, snf3 rgt2, recovered sensitivity to glucose when MTH1 was deleted, thus showing that glucose signalling may occur independently of Snf3 and Rgt2. A model for the possible mode of action of Snf3 and Rgt2 is presented.Keywords
This publication has 51 references indexed in Scilit:
- Glucose Signaling in Yeast Is Partially Mimicked by Galactose and Does Not Require the Tps1 ProteinMolecular Cell Biology Research Communications, 1999
- The molecular genetics of hexose transport in yeastsFEMS Microbiology Reviews, 1997
- Toward a functional analysis of the yeast genome through exhaustive two-hybrid screensNature Genetics, 1997
- Two glucose transporters in Saccharomyces cerevisiae are glucose sensors that generate a signal for induction of gene expression.Proceedings of the National Academy of Sciences, 1996
- A mutation affecting carbon catabolite repression suppresses growth defects in pyruvate carboxylase mutants from Saccharomyces cerevisiaeFEBS Letters, 1995
- New heterologous modules for classical or PCR‐based gene disruptions in Saccharomyces cerevisiaeYeast, 1994
- Trehalose‐6‐phosphate, a new regulator of yeast glycolysis that inhibits hexokinasesFEBS Letters, 1993
- A novel genetic system to detect protein–protein interactionsNature, 1989
- Two differentially regulated mRNAs with different 5′ ends encode secreted and intracellular forms of yeast invertaseCell, 1982
- An Examination of the Crabtree Effect in Saccharomyces cerevisiae: the Role of Respiratory AdaptationJournal of General Microbiology, 1979