State-of-the-Art Review: Usefulness of Laboratory Techniques for Evaluating Antithrombotic Efficacy of New Therapeutic Strategies

Publisher Website
Google Scholar
Abstract
New drugs and new therapeutic strategies are being introduced for the prevention and the management of cardiovascular and thromboembolic disorders. Extensive clinical studies and large-scale epidemiological investigations are conducted to demonstrate the efficacy of these therapeutic approaches. Laboratory assays are invaluable tools for this exploration. We discuss how the new understanding concerning the regulation of the coagulolytic equilibrium offers novel investigation tools. Parameters reflecting the activities of new drugs targeted to their impact site and presenting few side effects are available. In addition to global clotting methods and chromogenic substrate-based assays, introduction of immunoassays has allowed measurement of most of the molecular markers of hemostatic activation. We recommend use of a panel of markers exploring the endothelial damage, the blood cell involvement, the early coagulant pathways' activation (XIIa and VIIa), the thrombin-formation pathways, the fibrin formation, and the evaluation of the global fibrinolytic capacity. When related to the clinical end points, all these laboratory assays offer useful and reliable monitoring of new drugs. They contribute to the establishment of new therapeutic strategies. Key Words: Hemostasis—Activation—Antithrombotic—Therapy— Laboratory monitoring.