Transfection of cells with basic fibroblast growth factor and Kaposi fibroblast growth factor genes induce resistance to and receptor modulation of tumor necrosis factor
Open Access
- 18 September 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 372 (1) , 44-48
- https://doi.org/10.1016/0014-5793(95)00945-6
Abstract
Tumor necrosis factor (TNF) has been shown to inhibit the growth of some cell types and stimulate the proliferation of others by a mechanism that is not understood. In the present study, we investigated the effect of transfection of NIH‐3T3 cells with either the basic fibroblast growth factor gene (bFGF) or the kaposi FGF gene (K‐fgf) on the growth‐modulatory effects of TNF. Our results show that transformation of cells with either gene leads to resistance to the growth‐inhibitory effects of TNF. The K‐fgf gene was found to be a more potent inducer of cellular resistance than the bFGF gene. The cellular resistance correlated with the inhibition of TNF‐induced activation of phospholipase A2 and downmodulation of TNF receptors. Overall, our results indicate that both K‐fgf and bFGF play an important role in suppression of antiproliferative effects of TNF.Keywords
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