Structure of C-Type Virus Particles in Lymphocyte Cultures of Bovine Origin2

Publisher Website
Google Scholar
Abstract
Virus particles, primarily extracellular, were observed in 14 of 15 lymphocyte cultures from cows with hyperlymphocytosis. A detailed morphologic study was done on these particles after different fixations and negative staining with phosphotungstic acid. After glutaraldehyde and osmium fixation, the virion was roughly spherical (diameter ≈ 103 nm) and enclosed in an envelope that sometimes had a high electron density and quite often had surface projections. A core with a diameter of 40–90 nm was central and often separated from the intermediate membrane by a translucent space. After fixation with osmium or osmium followed by uranyl acetate treatment during dehydration, the intermediate membrane was not visible and the outline of the nucleoid was fuzzy. After negative staining, the clear surface projections were ≈ 114 Å long. Two morphologic features showed that the maturation of this virus particle differed from that described for the C-type particles: We found 1) particles with an electron-dense, central nucleoid (like mature C-type) still attached to the cell membrane and 2) no immature C-type particles. This suggests that the bovine particle matured when still attached to the cell membrane.

This publication has 0 references indexed in Scilit: