Elevation of -Aminobutyric Acid in Rat Brain with Hydroxylamine.

Abstract
The intraperitoneal injection of hydroxylamine (4.8 to 7.2 mg/kg body weight resulted in elevation of levels of gamma-aminobutyric acid in 8 cerebral areas of the rat. These elevated levels of gamma-aminobutyric acid were attained even after rats were pretreated with methylene blue to reduce the effects of methemoglobin formation. The effect of a single injection of hydroxylamine persisted for more than 5 hours. The potential value of this procedure for physiological study is discussed. The quantitative assay for gamma-aminobutyric acid was performed using an enzymatic technique.