Leaves, which had been killed in liquid air, were freeze dried and then fractionated by a nonaqueous method. Two fractions were obtained, one consisting of chloroplasts and the other of cytoplasm, vacuolar constituents, cell walls and residual chloroplasts. Calculation of the intracellular distribution of ascorbic acid based on the analysis of the two fractions revealed that 40 to 50% of the total ascorbic acid content of the cells is located within the chloroplasts. Since chloroplasts occupy less than 10% of the total volume of the cells, this high figure is direct evidence of an unequal distribution of ascorbic acid within the leaf cell.