Purification of the Azotobacter vinelandii nifV-encoded homocitrate synthase
- 1 September 1997
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 179 (18) , 5963-5966
- https://doi.org/10.1128/jb.179.18.5963-5966.1997
Abstract
The nifV gene product (NifV) from Azotobacter vinelandii was recombinantly expressed at high levels in Escherichia coli and purified. NifV is a homodimer that catalyzes the condensation of acetyl coenzyme A (acetyl-CoA) and alpha-ketoglutarate. Although alpha-ketoglutarate supports the highest level of activity, NifV will also catalyze the condensation of acetyl-CoA and certain other keto acids. E. coli cells in which a high level of nifV expression is induced excrete homocitrate into the growth medium.Keywords
This publication has 22 references indexed in Scilit:
- [8] Reassessment of Ellman's reagentPublished by Elsevier ,2004
- On carboxylate as a leaving group at the active site of Mo nitrogenase: electrochemical reactions of some MO and W carboxylates, formation of mono-, di- and tri-hydrides and the detection of an MoH2(N2) intermediatePolyhedron, 1994
- nifU Gene Product from Azotobacter vinelandii Is a Homodimer That Contains Two Identical [2Fe-2S] ClustersBiochemistry, 1994
- Cysteine desulfurase activity indicates a role for NIFS in metallocluster biosynthesis.Proceedings of the National Academy of Sciences, 1993
- Citrate substitutes for homocitrate in nitrogenase of a nifV mutant of Klebsiella pneumoniaeBiochemistry, 1990
- Diastereomer-dependent substrate reduction properties of a dinitrogenase containing 1-fluorohomocitrate in the iron-molybdenum cofactor.Proceedings of the National Academy of Sciences, 1990
- Biochemical and genetic analysis of the nifUSVWZM cluster from Azotobacter vinelandiiMolecular Genetics and Genomics, 1989
- Homocitrate is a component of the iron-molybdenum cofactor of nitrogenaseBiochemistry, 1989
- Identification of the V factor needed for synthesis of the iron-molybdenum cofactor of nitrogenase as homocitrateNature, 1987
- A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes.Proceedings of the National Academy of Sciences, 1985