Nuclear magnetic resonance study of d-TGGCCA in solution
Open Access
- 25 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (14) , 5927-5941
- https://doi.org/10.1093/nar/12.14.5927
Abstract
The 1H NMR spectrum of a self complementary deoxyhexanucleotide (d-TGGCCA) in water solution has been completely and unambiguously assigned by use of two-dimensional techoiques and phosphorus off-resonance decoupling experiments . The helix-coil transition has been monitored by following most of the non-exchangeable protons against temperature and was shown to be a cooperative dissociation-association process . Sequential fraying is sizable even at low temperature Considerations of coupling constants and of Nuclear Overhauser effects show the helix to be of the B-DNA typeKeywords
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