Isolation and Analysis of H-2 and Ia Alloantigens from Wild Mouse Strains
Open Access
- 1 July 1978
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 121 (1) , 141-148
- https://doi.org/10.4049/jimmunol.121.1.141
Abstract
Immunoprecipitation and SDS-PAGE analysis were used to examine H-2 and Ia antigens from mouse strains with wild-derived MHC haplotypes. Antisera raised against the wild-derived strains contained anti-H-2 and anti-Ia antibodies which precipitated antigen molecules readily distinguishable by a single assay procedure. The antibodies to wild strains were cross-reactive with standard laboratory haplotypes. Evidence supporting the similar genetic organization of wild and standard haplotypes was found, including isolation of separate H-2K and H-2D molecules from a wild-derived strain, and isolation of two separate Ia molecules from a wild-derived strain.This publication has 0 references indexed in Scilit: