Alternative conformations in Escherichia coli 16S ribosomal RNA.
- 1 June 1985
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 82 (11) , 3539-3542
- https://doi.org/10.1073/pnas.82.11.3539
Abstract
Partially denatured 16S rRNA from 30S ribosomes shows features of secondary structure in EM that correspond to the well accepted secondary structure model derived from chemical modification and phylogenetic data. However, a very different conformation is seen in precursor 16S rRNA sequences contained within 30S pre-rRNA transcripts: the major 5''-terminal loop is absent, and several additional quite stable large loops, symmetrically placed in the molecule, are present. Features of the alternative structure are also seen in mature 16S rRNA from E. coli and from 2 Bacillus species [B. subtilis and B. stearothermophilus] when heated in certain buffers. Microscopy thus reveals specific features of alternative conformations and their relative stabilities, suggesting a possible transition during ribosome formation.This publication has 16 references indexed in Scilit:
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