Collaborative clinical evaluation of the Autobac IDX system for identification of gram-negative bacilli

Publisher Website
Google Scholar
Abstract
The Autobac IDX system for rapid, semiautomated identification of gram-negative bacilli was compared with the identification methods in routine use in 4 laboratories. The study included 1515 organisms representing 30 spp. of enteric and nonenteric bacteria. Discrepancies between the results of the IDX system and routine methods were resolved by classical biochemical testing at a reference center. Overall, 98% of the organisms were correctly identified by the routine methods; 93% were correctly identified by the IDX systems. After adjustment for frequency of clinical occurrence of the organisms tested, the IDX system performed with 95% accuracy. Results with the IDX system were available in 3-6 h. Results with the comparative methods were available in 4-48 h. A wide variety of organisms, including oxidase-positive, oxidase-negative, fermentative and nonfermentative, were identified by a single system by using Autobac; .gtoreq. 3 systems were required to identify the 30 spp. by the comparative methods. Overall, the results indicate the Autobac IDX system is useful for the rapid identification of enteric and nonfermentative gram-negative bacilli.

This publication has 4 references indexed in Scilit: