In Vitro Selection of Calli Resistant to a Triazole Cytochrome-P-450-Obtusifoliol-14-Demethylase Inhibitor from Protoplasts of Nicotiana tabacum L. cv Xanthi
Open Access
- 1 July 1990
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 93 (3) , 1190-1195
- https://doi.org/10.1104/pp.93.3.1190
Abstract
The selection of biochemical mutants has been undertaken in order to elucidate regulatory and functional aspects of sterol biosynthesis in plants. 2-(4-Chlorophenyl)-3-phenyl-1-(1H-1,2,4- triazol-1-yl)-2,3-oxidopropane (LAB170250F), an experimental fungicide of the triazole family, was used as a selective agent. Indeed, this compound is a strong inhibitor of the cytochrome-P-450-obtusifoliol-14-demethylase in sterol biosynthesis. The selection strategy consisted of screening large populations of microcalli derived from ultraviolet-mutagenized protoplasts of Nicotiana tabacum L. cv Xanthi for resistance to a lethal concentration of LAB170250F. The best selective conditions were first determined, i.e. strength of the selection pressure as well as the time and duration of its application in the developmental process from protoplast to whole plant. Selection experiments resulted in the recovery of 40 resistant calli. These calli were divided into three classes according to the modification of their sterol content in response to LAB170250F. Some of these calli might be impaired in sterol biosynthesis, but most have a sterol profile identical to that of the control calli. This suggests that the toxic properties of LAB170250F are due to the parallel inhibition of sterol biosynthesis and of at least one additional unidentified target in the plant cell.This publication has 8 references indexed in Scilit:
- Genetic and biochemical evidence for multiple forms of acetolactate synthase in Nicotiana tabacumMolecular Genetics and Genomics, 1987
- The 14α-demethylation of obtusifoliol by a cytochrome P-450 monooxygenase from higher plants microsomesBiochemical and Biophysical Research Communications, 1986
- Effects of sterol manipulation on microsomal desaturase activities in Tetrahymena: With regard to thermal acclimationBiochemical and Biophysical Research Communications, 1983
- Effects of cycloartenol and lanosterol on artificial and natural membranesBiochemical and Biophysical Research Communications, 1980
- The functional importance of structural features of ergosterol in yeast.Journal of Biological Chemistry, 1978
- Isolation and partial characterization of a cholesterol-requiring mutant of Chinese hamster ovary cells.Proceedings of the National Academy of Sciences, 1977