Boolarra Virus: a Member of the Nodaviridae Isolated from Oncopera intricoides (Lepidoptera: Hepialidae)

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Abstract
A small RNA virus with a bipartite genome was isolated from O. intricoides. It was named Boolarra virus (BoV). The particle had a diameter of 30 nm, a sedimentation coefficient of 140S, and a buoyant density of 1.34 g/ml in CsCl. The capsid proteins were examined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and consisted of a major species of MW 38,000 and a minor one of MW 40,600. The particle contained 21.2% RNA which was divided between a 22S and a 17S species whose MW were 1.03 .times. 106 and 0.47 .times. 106, respectively. These properties were comparable to those of the Nodaviridae and established BoV as a member of the family. Serologically, BoV shared antigenic determinants with both Nodamura virus and black beetle virus.