PLASMODIUM-FALCIPARUM IN CONTINUOUS CULTURE - A NEW MEDIUM FOR THE INVITRO TEST FOR SULFADOXINE SENSITIVITY

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  • 1 January 1982
    • journal article
    • research article
    • Vol. 60  (3) , 423-426
Abstract
The sulfadoxine sensitivity of 2 strains of P. falciparum from Thailand, FCM2 and FCM5, was assessed using 2 types of culture medium, Waymouth formula and RPMI 1640. Growth of the parasite was completely inhibited by 0.5 mmol of sulfadoxine/l of Waymouth formula, whereas parasite growth in RPMI was not affected at this concentration. The apparent difference in drug sensitivity was caused by competition between 4-aminobenzoic acid was sulfadoxine. This hypothesis was further confirmed by the extent to which [14C]-sulfadoxine was incorporated into the infected erythrocytes.