HOST CELL REACTIVATION IN MAMMALIAN CELLS‐V. PHOTOREACTIVATION STUDIES WITH HERPES VIRUS IN MARSUPIAL AND HUMAN CELLS

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Abstract
Abstract— The survival of UV‐irradiated herpes simplex virus was determined in cultured Potoroo (a marsupial) and human cells under lighting conditions which promote photoreactivation. Photoreactivation was readily demonstrated for herpes virus in two lines of Potoroo cells with dose reduction factors of 0.7‐0.8 for ovan cells and 0.5‐0.7 for kidney cells. Light from Blacklite (near UV) lamps was more effective than from Daylight (mostly visible) lamps, suggesting that near UV radiation was more efficient for photoreactivation in Potoroo cells. The quantitative and qualitative aspects of this photoreactivation were similar to those reported for a similar virus infecting chick embryo cells. UV‐survhal curves for herpes virus in Potoroo cells indicated a high level of “dark” host cell reactivation. No photoreactivation was found for UV‐irradiated vaccinia virus in Potoroo cells. A similar photoreactivation study was done using special control lighting (Λ > 600 nm) and human cells with normal repair and with ceils deficient in excision repair (XP). No photoreactivation was found for UV‐irradiated herpes virus in either human cell with either Blacklite or Daylight lamps as the sources of photoreacti‐vating light. This result contrasts with a report of photoreactivation for a herpes virus in the same XP cells using incandescent lamps.

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