An Improved Technique for Fluorescence Staining of Fungal Nuclei and Septa.

Abstract
Nuclei and septa of fungal mycelium grown on glass slides coated with potato-dextrose agar stained very clearly with fluorescent Hoechst Dye 33258 in buffer of pH 7.8 and 10.5, respectively. The technique is simple, rapid, and gives excellent and clear staining.

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